VARS2 (NM_020442) Human Untagged Clone
CAT＃: SC318474
VARS2 (untagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2
CNY 7408.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | COXPD20; VALRS; VARS2L; VARSL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318474 representing NM_020442.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCTCATTTGCCTCTCGCCTCTTTTCGACCACCATTTTGGGGGCTGAGGCACTCACGGGGCCTCCCC AGGTTTCACTCCGTTTCTACACAGTCGGAGCCCCATGGATCTCCCATCTCCCGGAGGAACCGTGAAGCC AAACAGAAGCGCCTGCGAGAGAAGCAGGCGACTCTGGAGGCTGAGATAGCAGGGGAGAGCAAGTCACCT GCAGAATCCATTAAGGCCTGGAGGCCTAAGGAGTTAGTATTGTATGAAATCCCTACGAAACCCGGTGAA AAGAAAGATGTCTCTGGGCCCCTGCCTCCTGCATACAGCCCCCGATATGTTGAGGCTGCCTGGTACCCG TGGTGGGTACGAGAGGGCTTCTTCAAACCAGAATATCAGGCCCGGCTGCCCCAAGCTACAGGGGAGACC TTTTCCATGTGTATCCCACCTCCCAATGTCACTGGCTCCCTGCACATTGGCCACGCACTCACGGTGGCC ATACAGGATGCCCTCGTGCGCTGGCACCGGATGCGTGGGGATCAAGTGCTGTGGGTCCCTGGTTCAGAT CATGCAGGAATTGCTACACAAGCTGTGGTGGAGAAACAACTGTGGAAGGAACGGGGAGTGAGGAGACAT GAGCTGAGCCGGGAGGCCTTCCTTAGGGAGGTGTGGCAGTGGAAGGAGGCGAAAGGTGGAGAGATCTGT GAGCAGCTGCGAGCTCTGGGTGCCTCCCTGGACTGGGATCGAGAGTGTTTTACCATGGATGTTGGCTCC TCAGTGGCTGTGACTGAAGCTTTTGTGCGGCTCTACAAGGCGGGGTTGCTGTACCGGAACCATCAGCTT GTCAACTGGTCATGTGCTTTAAGATCAGCCATCTCGGACATTGAGGTGGAGAACCGGCCCCTGCCTGGC CACACACAGCTTCGACTGCCTGGCTGCCCCACCCCCGTGTCTTTTGGCCTCCTATTTTCTGTTGCCTTC CCCGTGGATGGAGAGCCTGATGCAGAGGTTGTGGTAGGAACCACAAGGCCAGAGACGCTGCCTGGAGAT GTGGCTGTGGCCGTTCATCCAGACGACTCGCGATACACACATCTACACGGGCGACAGCTTCGTCACCCC TTGATGGGGCAGCCTCTTCCCCTCATCACAGACTATGCTGTTCAGCCACATGTGGGCACGGGGGCAGTG AAGGTGACTCCAGCTCACAGTCCTGCCGATGCTGAGATGGGGGCCCGACATGGCTTGAGCCCCTTGAAT GTCATTGCGGAGGATGGGACCATGACCTCTCTCTGCGGGGACTGGCTGCAGGGTCTTCACCGGTTTGTG GCCCGGGAAAAGATAATGTCTGTGCTGAGTGAACGGGGCCTGTTCCGGGGCCTCCAGAACCACCCCATG GTACTGCCCATCTGCAGCCGTTCTGGGGATGTGATAGAATACCTGCTGAAGAACCAGTGGTTTGTCCGC TGCCAGGAAATGGGGGCCCGAGCTGCCAAGGCTGTGGAGTCGGGGGCCCTGGAGCTCAGTCCCTCCTTC CACCAGAAGAACTGGCAGCACTGGTTTTCCCATATTGGGGACTGGTGTGTCTCCCGGCAGCTGTGGTGG GGCCATCAGATTCCAGCCTACCTGGTTGTAGAGGACCATGCGCAGGGAGAAGAGGACTGTTGGGTGGTC GGGCGGTCAGAGGCTGAGGCCAGAGAGGTAGCAGCGGAACTGACAGGGAGGCCAGGGGCAGAGCTGACC CTGGAGAGGGATCCTGATGTCCTAGACACATGGTTTTCTTCTGCCCTGTTCCCCTTTTCTGCCCTGGGC TGGCCCCAAGAGACCCCAGACCTTGCTCGTTTCTACCCCCTGTCACTTTTGGAAACGGGCAGCGACCTT CTGCTGTTCTGGGTGGGCCGCATGGTCATGTTGGGGACCCAGCTCACAGGGCAGCTGCCCTTCAGCAAG GTGCTTCTTCATCCCATGGTTCGGGACAGGCAGGGCCGGAAGATGAGCAAGTCCCTGGGGAATGTGCTG GACCCAAGAGACATCATCAGTGGGGTGGAGATGCAGGTGCTGCAGGAAAAGCTGAGAAGCGGAAATTTG GACCCTGCAGAGCTGGCCATTGTGGCTGCAGCACAGAAAAAGGACTTTCCTCACGGGATCCCTGAGTGT GGGACAGATGCCCTGAGATTCACACTCTGCTCCCATGGAGTTCAGGCGGGCGACTTGCACCTGTCAGTC TCTGAGGTCCAGAGCTGCCGACATTTCTGCAACAAGATCTGGAATGCTCTTCGCTTTATCCTCAATGCT TTAGGGGAGAAATTTGTGCCACAGCCTGCTGAGGAGCTGTCTCCCTCCTCCCCGATGGATGCCTGGATC CTGAGCCGCCTTGCCCTGGCTGCCCAGGAGTGTGAGCGGGGCTTCCTCACCCGAGAGCTCTCGCTCGTC ACTCATGCCCTGCACCACTTCTGGCTTCACAACCTCTGTGACGTCTACCTGGAGGCTGTGAAGCCCGTG CTGTGGCACTCGCCCCGCCCCCTGGGGCCCCCTCAGGTCCTGTTCTCCTGCGCTGACCTCGGCCTCCGC CTCCTGGCCCCACTGATGCCCTTCCTGGCTGAAGAGCTCTGGCAGAGGCTGCCCCCCAGGCCTGGTTGC CCCCCTGCCCCCAGCATCTCGGTTGCCCCCTACCCCAGCGCCTGCAGCTTGGAGCACTGGCGCCAGCCA GAGCTGGAGCGGCGCTTCTCCCGGGTCCAAGAGGTCGTGCAGGTGCTAAGGGCTCTCCAAGCCACGTAC CAGCTCACCAAAGCCCGGCCCCGAGTGCTGCTGCAGAGCTCAGAGCCTGGGGACCAGGGCCTCTTCGAG GCCTTCTTGGAGCCCCTGGGCACCCTGGGCTACTGTGGGGCTGTGGGCCTGTTACCCCCAGGCGCAGCA GCTCCCTCCGGCTGGGCCCAGGCTCCACTCAGTGACACGGCTCAAGTCTACATGGAGCTGCAGGGCCTG GTGGACCCGCAGATCCAGCTACCTCTGTTAGCCGCCCGAAGGTACAAGTTGCAGAAGCAGCTTGATAGC CTCACAGCCAGGACCCCATCAGAAGGGGAGGCAGGGACTCAGAGGCAACAAAAGCTTTCTTCCCTCCAG CTGGAATTGTCAAAACTGGACAAGGCAGCCTCTCACCTCCGGCAGCTGATGGATGAGCCTCCAGCCCCA GGGAGCCCGGAGCTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_020442 |
| Insert Size | 3192 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020442.3 |
| RefSeq Size | 3622 bp |
| RefSeq ORF | 3192 bp |
| Locus ID | 57176 |
| UniProt ID | Q5ST30 |
| Domains | tRNA-synt_1 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis |
| MW | 118.4 kDa |
| Gene Summary | This gene encodes a mitochondrial aminoacyl-tRNA synthetase, which catalyzes the attachment of valine to tRNA(Val) for mitochondrial translation. Mutations in this gene cause combined oxidative phosphorylation deficiency-20, and are also associated with early-onset mitochondrial encephalopathies. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2014] Transcript Variant: This variant (2) contains alternate 5' exon structure, and it thus differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) is shorter at the N-terminus, compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211310 | VARS2 (Myc-DDK-tagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 7400.00 |
|
| RC211310L3 | Lenti ORF clone of Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 12540.00 |
|
| RC211310L4 | Lenti ORF clone of Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 12540.00 |
|
| RG211310 | VARS2 (tGFP-tagged) - Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 9000.00 |
|
| SC100467 | VARS2 (untagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 3736.00 |
