TMEM199 (BC033113) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C17orf32; MGC45714; transmembrane protein 199
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC102953 sequence for BC033113 edited (data generated by NextGen Sequencing) ATGGCGTCCTCTTTGCTTGCGGGCGAGCGATTGGTGCGTGCTTTGGGCCCCGGCGGGGAG CTGGAGCCAGAGCGGCTACCCCGAAAGCTGCGGGCCGAGCTTGAGGCCGCGCTGGGGAAG AAGCACAAGGGCGGTGATAGCTCCAGTGGCCCCCAACGCTTGGTTTCTTTCCGTCTCATC CGGGATCTGCACCAGCATCTGAGAGAAAGGGATTCCAAACTATACCTCCATGAGCTCCTA GAAGGCAGTGAAATCTATCTCCCAGAGGTTGTGAAGCCTCCACGGAACCCAGAACTAGTT GCCCGGCTGGAGAAGATTAAGATACAGCTGGCCAATGAGGAATATAAACGGATCACCCGC AACGTCACTTGTCAGGATACAAGACATGGTGGGACTCTCAGCGACCTGGGAAAGCAAGTG AGATCATTGAAGGCTCTGGTCATCACCATCTTCAATTTCATTGTCACGGTGGTTGCTGCC TTCGTCTGCACTTACCTTGGAAGCCAATATATCTTCACAGAAATGGCCTCGCGGGTGCTA GCTGCATTGATCGTCGCCTCTGTGGTGGGTCTGGCCGAGCTGTATGTCATGGTGCGGGCA ATGGAAGGCGAGCTGGGAGAACTGTAA Clone variation with respect to BC033113.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC033113 unedited GTCAGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTAGATATGG CGTCCTCTTTGCTTGCGGGCGAGCGATTGGTGCGTGCTTTGGGCCCCGGCGGGGAGCTGG AGCCAGAGCGGCTACCCCGAAAGCTGCGGGCCGAGCTTGAGGCCGCGCTGGGGAAGAAGC ACAAGGGCGGTGATAGCTCCAGTGGCCCCCAACGCTTGGTTTCTTTCCGTCTCATCCGGG ATCTGCACCAGCATCTGAGAGAAAGGGATTCCAAACTATACCTCCATGAGCTCCTAGAAG GCAGTGAAATCTATCTCCCAGAGGTTGTGAAGCCTCCACGGAACCCAGAACTAGTTGCCC GGCTGGAGAAGATTAAGATACAGCTGGCCAATGAGGAATATAAACGGATCACCCGCAACG TCACTTGTCAGTAGAGACCACGTTGGCCAGGCTGGTCTTGAACTCCCAGCCTCAAGGTGA TCCTCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAAGGATACAAGACATGGTGGGACT CTCAGCGACCTGNGAAAGCAAGTGAGATCATTGAAGGCTCTGGTCATCACCATCTTCAAT TTCATTGTCACGGTGGTTGCTGCCTTCGTCTGCACTTACCTTGGAAGCCCATATATCCTC ACAGAAATGGCCTCGCGGGTGCTAGCTGCATTGATCGTCGCCTCTGTGGTGGGTCTGGCC GAGCTGTATGTCATGGTGGCGGGCAATGAAAGGCGAGCTGGGAGAACCTGTACTGTTGCC NCATCATCAAGTCTAGAGGAGACTTGGGGGCTTCAGGCTCCATTTGCAGTCCCCGACTCA ATCACCCTTCAGATTTTTGGATTCAGCTCCATTTAGTCAGAGACCACCCCAGCCAGCTGT TGTTCTGTGGGGAGCCCTAACTTCTGGAAATCCCA
Restriction Sites	NotI-NotI
ACCN	BC033113
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC033113.1, AAH33113.1
RefSeq Size	1466 bp
RefSeq ORF	627 bp
Locus ID	147007
Protein Families	Transmembrane
Gene Summary	The protein encoded by this gene has been observed to localize to the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) and coat protein complex I (COPI) in some human cells. The encoded protein shares some homology with the yeast protein Vma12. Defects in this gene are a cause of congenital disorder of glycosylation, type IIp. [provided by RefSeq, Mar 2016]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.