MOSC2 (MARC2) (AL136931) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MOSC2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC106260 sequence for AL136931 edited (data generated by NextGen Sequencing) ATGGGCGCTTCCAGCTCCTCCGCGCTGGCCCGCCTCGGCCTCCCAGCCCGGCCCTGGCCC AGGTGGCTCGGGGTCGCCGCGCTAGGACTGGCCGCCGTGGCCCTGGGGACTGTCGCCTGG CGCCGCGCATGGCCCAGGCGGCGCCGGCGGCTGCAGCAGGTGGGCACCGTGGCGAAGCTC TGGATCTACCCGGTGAAATCCTGCAAAGGGGTGCCGGTGAGCGAGGCTGAGTGCACGGCC ATGGGGCTGCGCAGCGGCAACCTGCGGGACAGGTTTTGGCTGGTGATTAAGGAAGACGGA CACATGGTCACTGCCCGGCAGGAGCCTCGCCTCGTGCTCATCTCCATCATTTATGAGAAC AACTGCCTGATCTTCAGGGCTCCAGACATGGACCAGCTGGTTTTGCCTAGCAAGCAGCCT TCCTCAAACAAACTCCACAACTGCAGGATATTTGGCCTTGACATTAAAGGCAGAGACTGT GGCAATGAGGCAGCTAAGTGGTTCACCAACTTCTTGAAAACTGAAGCGTATAGATTGGTT CAATTTGAGACAAACATGAAGGGAAGAACATCAAGAAAACTTCTCCCCACTCTTGATCAG AATTTCCAGGTGGCCTACCCAGACTACTGCCCGCTCCTGATCATGACAGATGCCTCCCTG GTAGATTTGAATACCAGGATGGAGAAGAAAATGAAAATGGAGAATTTCAGGCCAAATATT GTGGTGACCAGCTGTGATGCTTTTGAGGAGGCTTCAGCAACCAGGAGGGATTGA Clone variation with respect to AL136931.1 297 t=>c;318 a=>g;360 t=>c;730 g=>a 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL136931 unedited GGGGCCAANCCATCCTCCCNNNNNGGGTTAGATTTGTAAACAATCTTATAGGCGGCCGCG AATTCGCACGAGTCGCTCCCCGGCTCTCCAGGTACCCCCGCCGCTGTCTGCCTGTCTTCC TCCATTACCGCGCAGGCTTGGTCACCGCATTAAGGCATTCCCGCTCTCCGCGGAACTGCT CTGCCGTCTCGGCGGTGAAAGTGTGAGAGGGTCCGTAGTTGGGTCAACTTTGACTCCTCT CGCCTGCCCGGATCCTTAAGGGCCTCCTCGTCCTCCCGGTCTCCGGTCGCTGCCGGGTCT GTGCGCCGGTCCGCGCCCGCCCTCGCTCTGCCATGGGCGCTTCCAGCTCCTCCGCGCTGG CCCGCCTCGGCCTCCCAGCCCGGCCCTGGCCCAGGTGGCTCGGGGTCGCCGCGCTAGGAC TGGCCGCCGTGGCCCTGGGGACTGTCGCCTGGCGCCGCGCATGGCCCAGGCGGCGCCGGC GGCTGCAGCAGGTGGGCACCGTGGCGAAGCTCTGGATCTACCCGGTGAAATCCTGCAAAG GGGTGCCGGTGAGCGAAGCTGANTGCACGGCCATGGGGCTGCGCAGCGGCACCTGCGGAC AGGTTTTGGCTGGTGATAAAGAAGACGGACACATGGTCACTGCCCCGCANGAGCCTCGNC TCGTGCTCATCTCCATCATTTATGAGAACAACTGCCTGATCTTCANGGCTCCAGACATGG GACAGCTGGGTTTTGCCTAGCAAGCAGCCTTTCCTCAACAAACTNCACCACTGCAGGATA ATTGGCCCTGACATTAAAGGCAGAGACTGTGGCCAATGAGCACCTAAGTTGGTCACCCAC CTCTTGAAAACTGAGCGATAAAAGGGTTCCATTTGAGACAACCATGAGGGGAGAACATCA GGAAAACTTCTCCCCACTCTGATAGGATTTCCAGTGGCCTACCCAACTACTGCCCGTTCC TGACATGAAAAATGCTCCCTGTAAATATGAACCCGGATGGAAAGAAAGGAAATGGGAAAT TCG
Restriction Sites	NotI-NotI
ACCN	AL136931
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL136931.1, CAB66865.1
RefSeq Size	1350 bp
RefSeq ORF	1350 bp
Locus ID	54996
Domains	MOSC_N
Protein Families	Transmembrane
Gene Summary	The protein encoded by this gene is an enzyme found in the outer mitochondrial membrane that reduces N-hydroxylated substrates. The encoded protein uses molybdenum as a cofactor and cytochrome b5 type B and NADH cytochrome b5 reductase as accessory proteins. One type of substrate used is N-hydroxylated nucleotide base analogues, which can be toxic to a cell. Other substrates include N(omega)-hydroxy-L-arginine (NOHA) and amidoxime prodrugs, which are activated by the encoded enzyme. Multiple transcript variants encoding the different isoforms have been found for this gene. [provided by RefSeq, Sep 2016]

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