FAM83D (BC001068) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C20orf129; CHICA; dJ616B8.3
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC001068, the custom clone sequence may differ by one or more nucleotides CGAGTCCGAGCGCCGCCATGGCTCTGCTGTCCGAGGGCCTGGACGAGCTGCCCGCCGCCTGCCTGTCGCC GTGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCACGGCGCCTGGCTCTGGAGGAGCTGGTGGCG GGCGGCCCCGAAGCCTTCGCGGCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGG TGCACGCCATTCTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGGCGGCGGCGGCGGCCGAGGA CTCGTTCGGCTCCTCGCACGACTGCTCTTCGGGCACCTACTTCCCCGAGCAGTCGGACCTGGAGCCACCG CTGTTGGAGCTTGGCTGGCCCGCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCGAGACGCACT TCCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGCAAGGACGCTCTGCGCCAGCAGCTCCGCTC GGCGCGAGAGGTGATTGCAGTGGTCATGGACGTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAA ATATGCAGGAAACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTCCTCTCTCAATTTTTGGATA TGTGCATGGATCTGAAAGTTCATCCTGAACAGGAAAAGTTAATGACAGTTCGGACTATCACAGGAAATAT CTACTATGCAAGGTCAGGAACTAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATTGATGGCATC CGCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGGCAAATTAAACAGCAGTAACTTGGTAATTC TGTCTGGCCAAGTGGTTGAACACTTTGATCTGGAGTTCCGAATCCTGTATGCCCAGTCCAAGCCCATCAG CCCCAAACTCCTGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCACCAACCGAAAACCACAGTCC AAGGAGCTCACCCTGGGCAACCTGCTGCGGATGCGGCTGGCTAGGCTGTCAAGTACTCCCAGGAAGGCGG ACCTGGACCCAGAGATGCCCGCAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTCCTCTACTGT TAGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCCAGAGCAGAAAGGCCATTGACGCTGCCACT CAAACAGAGCCAGGAGAGGAGATGCCAGGGCTGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCACCA CAGCATGTGCTGGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTCAAACCACGATTTGGTCCAG ATCGACCACTACTCAGACTGACATGGATGAGAACATTCTCTTTCCTCGAGGAACTCAATCTACAGAAGGG TCACCAGTCTCAAAAATGTCTGTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGTCTTCCCAAG GCTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGAACCACTGACTTCCACAATCCTGGCTATCC CAAGTACCTGGGCACCCCCCACCTGGAACTGTACTTGAGTGACTCACTTAGAAACTTGAACAAAGAGCGG CAATTCCACTTCGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATGCTGTCAAGGAGAACACTCT TTACTGAAAACCACCTTGGCCTTCATTCTGGCAATTTCAGCAGAGTTAATTTGCTTGCTGTTAGAGATGT AGCACTTTATCCTTCCTATCAGTAACTGCTCCGTGTTCAGACTCCTGGTTTCTTCCAGGCTTACAGTGGA CATCATCAGCTTCCTGCTTTAAAAAATATCTTATGTCCCTAATTGCCTTTCTTTTACCTGACTTTGTCAC CTTTGTTGTCTTTGAATTCTTTAGGCTGCATATTATTTTACATGCTTTGTTTTGTCATGTATATACCAGG TATTGGTTTTATGGTTTAAACACTATGGATACAGGGGTTTGTTTTGCACAATTTTAATAGTCATGCACTA CATAATGATGTTTTGGTCGATGACAGACCACGTATATGTTGGCAGTCTCATAAGATTATAATACTGTATT TTTACTATACCTTTTCTATGTTTAGATACAAATACCATTATGTTACAGTTGCCTACAGTATTCAGTGCAG TAACATGATGTACAGGTTTGTAGCCTGTTTTGCATTTTTCTTAGGTTGTATGCTCTTCTGTTTTAAAGGT TTGAATCACCAGCATTTTTGTGATCAAAATCCTATTTAGAAAAAATAAAACTACTTTCTGTTTAAAAAAA AAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC001068 unedited TCATTTTGTATACGACTCACTATAGGCGGCCCGCGAATTCGCACGAGGCCATGGCTCTGC TGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTGCCTGTCGCCGTGCGGGCCGCCCAACC CGACCGAGCTGTTCAGCGAGTCACGGCGCCTGGCTCTGGAGGAGCTGGTGGCGGGCGGCC CCGAAGCCTTCGCGGCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATG AGGTGCACGCCATTCTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGGCGGCGG CGGCGGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCGGGCACCTACTTCCCCG AGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCTTGGCTGGCCCGCCTTCTACCAGGGCG CCTACCGCGGCGCCACGCGTGTCGAGACGCACTTCCAGCCCCGCGGCGCTGGCGAAGGTG GCCCCTACGGCTGCAAGGACGCTCTGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTG CAGTGGTCATGGACGTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAATATGCA GGAAACAGGGAGTTCGCTGTGTATATCCTTCTGGACCAGGCTCTCCTCTCTCATTTCTGG ATATGTGCATGGATCCTGAAAGCTCATCCTGAACAGGAAAAGCTAACGACCGGTTCGGAC CATCACAGGAAATATCTACTATGCACGGCCAGGAACTAACATATTGGGGAAAGGGTCCCG AAATGTCCAGGTTGATTGGTGTGATTAGGGTGGAAACCGGTTCCTACGGAACCGCCCCGG CTCGTATCACTCCCTCGTCAAAACCTCGT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC001068 unedited NGGAAATACTGTGCACCGCGGCCGCAATCTACGAGCGAGTTTTTTTTTTTTTTTTTTTTT TTAAACAGAATTTTGTTTTATTTTTTTTAAATAGGATTTTGATCACAAAAATGCTGGTGA TTCAAACCTTTAAAACAGAAGAGCATACAACCTAAGAAAAATGCAAAACAGGCTACAAAC CTGTACATCATGTTACTGCACTGAATACTGTAGGCAACTGTAACATAATGGTATTTGTAT CTAAACACAGAAAAGGTATAGTAAAAATACAGTATTATAATCTTATGAGACTGCCAACAT ATACGTGGTCTGTCATTGACCAAAACATCATTATGTAGTGCATGACTATTAAAATTGTGC AAAACAAACCCCTGTATCCATAGTGTTTAAACCATAAAACCAATACCTGGTATATACATG ACAAAACAAAGCATGTAAAACAATATGCAGCCTAAAGAATTCAAAGACAACAAAGGTGAC AAAGTCAGGTAAAAGAAAGGCAATTAGGGACATAAGATATTTTTTACAGCAGGAAGCTGA TGATGTCCACTGTACGCCTGGAAGAAACCAGGAGTTTGAACACGGTACACTTACTGATAG GAAGGATAAAGTGCTACATTTTTACCAGTAAGCAAATTAACTCTGCTGAAATTGCCAGAC TGACGGCCAAGGCGGCTTTTCAGTAAAGAGTGTTTTCCTTTGACAGCACACCCCGCATGT GGCTTGACCCGGCACCTTGAACCCAGCGAAGCGGCAATTGCCGCTTTTCTGTTCACGCTT ATAAGTGAGTCACTCAAGCACAGTTCCAGGTCGGGGGGTGCCCAGTTCCTTGGGCATTAC CAGAATTGCGGAATTTAAGGGTCCTTGAGGAAGCGGGAAAAACAGAGGAGCTTGCCCAAA TACCTTGGCAAAACCACACGCGCAACAC
Restriction Sites	NotI-NotI
ACCN	BC001068
Insert Size	2320 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC001068.1, AAH01068.1
RefSeq Size	2333 bp
RefSeq ORF	555 bp
Locus ID	81610
Protein Families	Druggable Genome
Gene Summary	Probable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC (PubMed:24344117). May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade (PubMed:25646692). May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22.[UniProtKB/Swiss-Prot Function]

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