ZWILCH (NM_017975) Human Untagged Clone
CAT＃: SC113827
ZWILCH (untagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1
CNY 1200.00
CNY 9600.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hZwilch; KNTC1AP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113827 sequence for NM_017975 edited (data generated by NextGen Sequencing)
ATGTGGGAGCGGCTGAACTGCGCAGCAGAGGACTTTTATTCTCGTCTCCTTCAGAAATTT AATGAAGAAAAGAAAGGAATCCGTAAAGACCCATTTCTCTATGAGGCTGATGTCCAAGTG CAGTTGATCAGCAAAGGCCAACCAAACCCTTTGAAAAATATTCTAAATGAAAATGACATA GTATTCATAGTGGAAAAAGTGCCTTTAGAAAAGGAAGAAACAAGTCATATTGAAGAACTT CAATCTGAAGAAACTGCCATATCTGATTTCTCTACTGGCGAAAATGTTGGACCACTTGCT TTACCAGTTGGGAAGGCAAGGCAGTTAATTGGACTTTACACCATGGCTCACAATCCTAAT ATGACCCATTTGAAGATTAATCTGCCAGTTACTGCCCTTCCTCCCCTTTGGGTAAGATGT GACAGTTCAGATCCTGAAGGTACTTGTTGGCTAGGAGCTGAGCTTATCACAACAAACAAC AGCATTACAGGAATTGTCTTATATGTGGTCAGTTGTAAAGCTGATAAAAATTATTCTGTA AATCTTGAAAACCTAAAAAATTTACACAAGAAAAGACATCACTTGTCTACTGTAACATCC AAAGGCTTTGCCCAGTATGAGCTCTTTAAGTCCTCTGCCTTGGATGATACAATCACAGCA TCACAAACTGCGATCGCTTTGGATATTTCCTGGAGTCCTGTGGATGAGATTCTTCAAATC CCTCCACTCTCTTCAACTGCAACTCTGAATATTAAAGTGGAATCAGGAGAGCCCAGAGGT CCTTTGAATCATCTCTACAGAGAACTGAAATTTCTTCTTGTTTTGGCTGATGGTTTGAGG ACTGGTGTCACTGAATGGCTCGAGCCCCTGGAAGCAAAATCTGCTGTTGAACTTGTTCAG GAATTTCTGAATGACTTAAATAAGCTGGATGGATTTGGTGATTCTACAAAAAAAGACACT GAGGTTGAGACCTTGAAGCATGACACTGCTGCAGTCGATCGTTCCGTCAAGCGTCTTTTC AAAGTTCGGAGTGATCTTGATTTTGCTGAGCAACTGTGGTGCAAAATGAGCAGTAGTGTG ATTTCATACCAAGACTTGGTGAAGTGTTTCACATTGATCATCCAGAGTCTACAACGTGGT GATATACAGCCATGGCTCCATAGTGGAAGTAACAGTTTACTAAGTAAGCTCATTCATCAG TCTTATCATGGAACCATGGACACAGTTTCTCTCAGTGGGACTATTCCAGTTCAAATGCTT TTGGAAATTGGTTTGGACAAACTAAAGAAAGATTATATCAGTTTTTTCATAGGTCAGGAA CTTGCATCTTTGAATCATTTGGAATACTTCATTGCTCCATCAGTAGATATACAAGAACAG GTTTATCGTGTCCAAAAACTCCACCATATTCTAGAAATATTAGTCAGTTGCATGCCTTTC ATTAAATCTCAACATGAACTCCTCTTTTCTTTAACACAGATCTGCATAAAGTATTACAAA CAAAATCCTCTTGATGAGCAACACATTTTTCAGCTGCCAGTCAGACCAACTGCTGTAAAG AACTTATATCAAAGTGAGAAGCCACAGAAATGGAGAGTGGAAATATATAGTGGTCAAAAG AAGATTAAGACAGTTTGGCAACTGAGTGACAGCTCACCCATAGACCATCTGAATTTTCAC AAACCTGATTTTTCGGAATTAACACTAAACGGTAGCCTGGAAGAAAGGATATTCTTTACT AACATGGTTACCTGCAGCCAGGTGCATTTCAAGTGA Clone variation with respect to NM_017975.3 >OriGene 5' read for NM_017975 unedited
TACCCGACTTCTGTGGGGCGGAAAACCATTCGGCACGAGGGGGATGTGGGATCGGCTGAA CTGCGCAGCAGAGGACTCTTATTCTCGTCTCCTTCACCTTTAGAAAAGGAAGAAACAAGT CATATTGAAAAACTTCAATCTGAAGAAACTGCCATATCTGATTTCTCTACTGGCGAAAAT GTTGGACCACTTGCTTTACCATTCGTGATTGCAAGGCAGTTAATTGGACTTTACACCATG GCTCACAATCCTAATATGACCCATTTGAAGATTAATCTGCCAGTTACTGCCCTTCCTCCC CTTTGGGTAAGATGTGACAGTTCAGATCCTGAAGGTACTTGTTGGCTAGGAGCTGAGCTT ATCACAACAAACAACAGCATTACAGGAATTGTCTTATATGTGGTCAGTTGTAAAGCTGAT AAAAATTATTCTGTAAATCTTGAAAACCTAAAAAATTTACACAAGAAAAGACATCACTTG TCTACTGTAACATCCAAAGGCTTTGCCCAGTATGAGCTCTTTAAGTCCTCTGCCTTGGAT GATACAATCACAGCATCACAAACTGCGATCGCTTTGGATATTTCCTGGAGTCCTGTGGAT GAGATTCTTCANATCCCTCCACTCTCTTCAACTGCAACTCTGAATATTAAAGTGGAATCA GGAGAGCCCAGAGGTCCTTTTGATCATCTCTACAGAGAACTGANATTTCTTCTTGTTTTG GCTGATGGTTTGAGGACTGGTGTCACTGAATGCCTCGAGCCCCTGGAAGCAAATCTGCTG TTGAACTTGTTCAGAATTCTGATGACTTAAATAGCTGAATGGATTTGTGATTCTACAAAA AGAACTGAGGTGAAACTTGAGCATGACCTGCTGCAGTCACGTT >OriGene 3' read for NM_017975 unedited
CTCTCTTTATGTGGTAGCAAANATGGAAGTGCGAGACCAAATCTTACCAAACTAGCTATT TTTACAGGCCAATAAAGCAACATGCAATCCCCCTCAACAAATTTAAATAATCAGGCAATA CTAAGAATGTATATTCCATTAAACTAAAATAAACAAGGTTGAAATGTGGTACAGAATTCA CTGATGAGCCTGTGAACTCCACGTGAGGATGTCCAGTGCCTTATTTATCTCAGTAACCAG AGTACCCAGCACACAAGATAAAAGTGGGTATTACCTAAGTGGCCACTATTTTATTAATAA TGCACATAACATATGCTTATCATTAACTCTTAAAAAGATTATTATTTAACTATACAAGAA CTACCATACAACATTTCAACATACAAGTTCCTATACTTCTTAAGATACACAATAATATTT TGAAAGATTGGAAATGTTTCCCAAGGACTTTCCTATCACAAATCCCCCCCAGTAAAAATT ATAGGAACTGTGGAATATGGCTGCTAATATTCTGAGATGAATTTAGAAAGTATAAGTATT GAGAGATGGACTTTATTATAGCACCTGTAGAAGAACCAAATTCAGGCATTTATCCCATAT TCTCTGAAATATTTACAAAGCTCTTATAACATTTTCAAATAACATTTAATTTACTAAGTT CTATTTCATGGCAAATAAGAATCAGAAAATTTGAGGATGCTATTTTTTAAAGTTTCCCAA TACAGTTAAAGCTTTTGGGATTAAGCTGTATTTACTAAAACGATCCCTGTCTCATTTGTG GCGCCCGTTCTCTTTGTCATCCCGAGCCCTTGCTCAGCACCATGCAATGGTACTGAAATC GTTGGACCCACTGGCTGCGTCCTCTGTGACTCAAACTGCTGTCTATTAAGCTCCTCCTGA GAAGCATAGCTAAACAGGATTTCATATCTGGAGTCTCCAAGAAAAACTCCAGTTCTTTCT CCCTTTTCTGGTTGAGGGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_017975 |
| Insert Size | 2930 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017975.1, NP_060445.1 |
| RefSeq Size | 1670 bp |
| RefSeq ORF | 405 bp |
| Locus ID | 55055 |
| UniProt ID | Q9H900 |
| Gene Summary | Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213970 | ZWILCH (Myc-DDK-tagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 |
CNY 4400.00 |
|
| RC213970L3 | Lenti-ORF clone of ZWILCH (Myc-DDK-tagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 |
CNY 7130.00 |
|
| RC213970L4 | Lenti-ORF clone of ZWILCH (mGFP-tagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 |
CNY 7130.00 |
|
| RG213970 | ZWILCH (tGFP-tagged) - Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 |
CNY 5700.00 |
|
| SC318018 | ZWILCH (untagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 |
CNY 9600.00 |
