MYO1E (NM_004998) Human Untagged Clone
CAT＃: SC309978
MYO1E (untagged)-Human myosin IE (MYO1E)
CNY 10450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FSGS6; HuncM-IC; MYO1C |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC309978 representing NM_004998.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGAAGCAAAGGTGTCTACCAGTACCACTGGCAAAGCCACAATGTCAAGCACAGTGGTGTGGACGAC ATGGTGCTACTGTCCAAGATCACAGAGAACTCCATCGTGGAGAATCTGAAGAAGAGATACATGGATGAC TACATTTTTACATATATAGGATCTGTATTAATCTCAGTCAACCCTTTCAAGCAGATGCCATATTTTGGG GAAAAGGAAATTGAAATGTACCAAGGAGCGGCACAGTATGAAAACCCACCACATATCTATGCCCTTGCA GATAATATGTACAGAAACATGATCATTGACAGAGAGAACCAGTGCGTCATTATCAGTGGTGAAAGTGGT GCTGGAAAAACAGTGGCTGCCAAATATATCATGAGCTACATCTCCAGAGTGTCTGGAGGAGGGACCAAA GTCCAGCACGTGAAGGACATTATCCTGCAGTCCAACCCGCTGCTGGAGGCCTTCGGGAACGCCAAGACC GTCCGGAACAACAACTCCAGCCGATTTGGAAAATACTTTGAAATCCAGTTCAGTCCAGGTGGGGAACCA GATGGTGGAAAGATCTCCAACTTCCTTCTGGAAAAATCTAGGGTGGTGATGAGGAACCCAGGAGAGCGG AGTTTTCACATATTTTACCAGCTCATCGAGGGCGCCTCTGCAGAGCAGAAACACAGCCTTGGCATCACC AGCATGGACTATTATTACTACCTGAGCCTCTCGGGCTCATACAAGGTTGATGACATTGACGACAGGCGG GAGTTTCAGGAAACTCTGCACGCCATGAATGTGATTGGGATCTTTGCAGAAGAGCAAACGCTGGTGTTG CAGATAGTGGCGGGTATTCTCCACCTGGGAAACATCAGCTTCAAAGAAGTTGGCAACTACGCGGCTGTG GAGAGTGAAGAGTTTTTAGCTTTTCCTGCATATCTGCTAGGGATAAACCAGGACCGGTTGAAAGAAAAG CTAACAAGCCGGCAGATGGATAGCAAGTGGGGAGGCAAATCCGAATCCATCCACGTGACCCTCAACGTA GAGCAGGCCTGTTACACCCGGGATGCGCTCGCCAAGGCCCTGCACGCCCGGGTCTTTGATTTCTTGGTA GATTCCATCAATAAAGCCATGGAGAAAGACCATGAAGAATACAACATTGGCGTCCTAGACATCTATGGC TTTGAAATATTCCAGAAAAATGGCTTTGAACAGTTTTGTATCAATTTTGTTAATGAAAAACTGCAGCAG ATTTTTATTGAACTGACATTAAAGGCAGAACAGGAAGAATATGTTCAAGAGGGAATAAGATGGACACCC ATTGAGTACTTTAATAATAAAATCGTATGTGACCTCATAGAGAACAAAGTGAACCCTCCTGGCATCATG AGCATCCTGGATGACGTGTGCGCCACGATGCATGCGGTGGGTGAGGGGGCAGATCAGACGCTGCTCCAG AAACTTCAGATGCAGATTGGGAGTCATGAGCACTTCAACAGTTGGAACCAAGGCTTCATCATTCATCAT TATGCTGGGAAGGTATCCTATGACATGGATGGCTTTTGTGAAAGGAACCGGGATGTGCTTTTTATGGAT CTCATCGAGCTTATGCAGAGCAGCGAGCTGCCTTTCATAAAGTCTTTATTTCCGGAAAATCTGCAGGCT GACAAGAAAGGGCGCCCAACTACTGCCGGAAGCAAAATAAAGAAACAAGCCAATGACCTTGTGAGCACC CTGATGAAATGTACGCCCCACTACATTCGCTGCATCAAGCCAAACGAAACCAAGAAGCCCAGAGACTGG GAGGAAAGCAGGGTAAAGCATCAAGTCGAATATTTGGGTCTGAAAGAGAACATTCGAGTGAGAAGAGCT GGCTATGCCTATCGGCGCATCTTCCAAAAATTCCTACAGAGGTATGCCATTCTGACCAAAGCCACCTGG CCTTCTTGGCAGGGAGAGGAGAAGCAAGGCGTCCTGCACCTGCTGCAGTCGGTCAACATGGACAGCGAC CAGTTCCAGCTGGGGAGGAGTAAAGTGTTCATCAAAGCCCCCGAGTCTCTATTTCTTTTAGAAGAGATG AGAGAGAGAAAGTATGATGGGTATGCTCGAGTGATACAGAAATCATGGAGGAAATTCGTGGCCCGGAAG AAATACGTTCAAATGAGAGAAGAAGCCTCAGACCTCTTATTGAACAAGAAGGAGAGAAGGAGAAACAGT ATTAACAGGAACTTTATAGGGGATTATATTGGGATGGAAGAGCACCCAGAACTCCAGCAGTTCGTGGGC AAGAGGGAGAAGATTGATTTCGCAGACACAGTCACCAAGTATGACAGGAGGTTCAAGGGTGTAAAGCGA GACCTGCTCCTTACCCCAAAGTGCTTGTACTTAATCGGACGAGAAAAAGTCAAACAGGGCCCAGACAAG GGCCTGGTGAAAGAAGTCCTGAAGCGGAAAATCGAGATAGAACGGATCTTGTCTGTGTCCCTCAGTACT ATGCAGGATGACATTTTTATTCTCCATGAGCAAGAGTATGACAGTTTGCTTGAATCTGTCTTCAAAACT GAATTCCTAAGCCTCTTAGCAAAGCGTTACGAGGAGAAGACCCAGAAGCAACTACCTCTGAAATTCAGC AATACGCTTGAACTGAAGTTGAAAAAGGAAAACTGGGGCCCCTGGAGTGCAGGGGGCTCCCGGCAAGTG CAGTTCCACCAAGGGTTTGGGGACCTGGCTGTCCTCAAGCCCAGTAACAAAGTGCTGCAGGTCAGCATC GGACCTGGACTGCCCAAGAACTCCCGTCCTACCAGAAGGAACACTACCCAAAATACAGGTTATTCCAGT GGGACTCAAAATGCCAACTACCCAGTGAGAGCTGCCCCTCCTCCCCCAGGATACCATCAGAACGGAGTC ATCAGAAACCAGTATGTGCCATATCCCCATGCTCCTGGAAGCCAGAGGTCCAATCAGAAAAGCCTGTAC ACCTCCATGGCCCGCCCGCCCTTGCCTCGGCAGCAGTCTACCAGTTCAGACCGAGTGTCACAGACGCCA GAGAGCCTGGATTTCCTCAAGGTCCCGGACCAGGGAGCTGCAGGGGTCAGGAGACAAACAACCAGTCGG CCTCCCCCAGCAGGGGGCAGACCCAAGCCCCAGCCCAAGCCCAAGCCTCAGGTGCCACAGTGCAAGGCT TTGTATGCCTATGACGCTCAGGACACAGACGAACTCAGCTTTAATGCCAATGACATTATTGATATTATC AAAGAAGATCCTTCTGGCTGGTGGACGGGTCGACTACGAGGCAAGCAGGGCCTGTTCCCCAACAACTAT GTGACCAAGATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_004998 |
| Insert Size | 3327 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004998.3 |
| RefSeq Size | 5125 bp |
| RefSeq ORF | 3327 bp |
| Locus ID | 4643 |
| UniProt ID | Q12965 |
| Domains | IQ, SH3, myosin_head |
| MW | 127.1 kDa |
| Gene Summary | This gene encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. Class I myosins are characterized by a head (motor) domain, a regulatory domain and a either a short or long tail domain. Among the class I myosins, this protein is distinguished by a long tail domain that is involved in crosslinking actin filaments. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in this gene are the cause of focal segmental glomerulosclerosis-6. This gene has been referred to as myosin IC in the literature but is distinct from the myosin IC gene located on chromosome 17. [provided by RefSeq, Jan 2012] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210513 | MYO1E (Myc-DDK-tagged)-Human myosin IE (MYO1E) |
CNY 7712.00 |
|
| RC210513L1 | Lenti ORF clone of Human myosin IE (MYO1E), Myc-DDK-tagged |
CNY 10112.00 |
|
| RC210513L2 | Lenti ORF clone of Human myosin IE (MYO1E), mGFP tagged |
CNY 7600.00 |
|
| RC210513L3 | Lenti ORF clone of Human myosin IE (MYO1E), Myc-DDK-tagged |
CNY 7600.00 |
|
| RC210513L4 | Lenti ORF clone of Human myosin IE (MYO1E), mGFP tagged |
CNY 7600.00 |
|
| RG210513 | MYO1E (tGFP-tagged) - Human myosin IE (MYO1E) |
CNY 9312.00 |
