IRF1 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA	RFP-BSD
Symbol	IRF1
Locus ID	3659
Kit Components	KN203500G1, IRF1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TTAATTCCAACCAAATCCCG KN203500G2, IRF1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AAGTACATGGGTTAAAACAG KN203500RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. Homologous arm and RFP-BSD sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet TACGGAGACT GCGGGAAGAG GCGCCACAGG TGTTCCTTGG GCCACTTCTC CAGAGGAGGG GAAACCGGGC CGGAAGGGTT AGCGTCCTGG TCTTAGCGTT GTGGGCGCTG TGGCTGTCAG GAAGGCGTAG AATGGATTCA GGGGGGCGGG AGGGGGCTGT TCAGGGTGAC GGCTAGCCCT TTGCTAGCTA GTGGTTACAA CTCAAGTCAA GGGAATTTCT TCTTGGCATC AAGCAAAAGA AGTCCCTCCC TTCCCAAAGG ATTTGAATTT TGAGCGAAAA GTTCTGAAAT TAGGGTATCT GTGCATTTTG TCTCTTTTCC TGCATATGAA TCCTGAAGCC ATCACTTGCA TGCCTGTCTC CTCCAGAGAC TGGCTGGGAG GGGCTGAAGG AAGGGGCAAA AGCATTTTTG CCTAAGATGC TGAAAAAATT TGGAGAGCAG TTTTATTCCA GCGCAGCTCC CCTCCGCACT GAGTGTAGTA CCTAGCAGCT GGCTGAGGTG AGGGGAGGGT AACTAAGTGA CCTCGGGTGG GGCAGGTCAC TGCCCAGGTA CTGTTCAACA GATTCCAGAC TGGAGCCTCT GTGTTCTCTT TACAGCCAAC GATGCAGCTC AAAAAGGGAA GTGATAACGG GACAAGCAGG TGTTTCTCCC AGTGGGTCCT GCATGCAGGG AGTGTGCACG GCCCAGCCTG GGCCTCACTT GCATGACTCC TGCCTTCTTC CCTTCTTGAG GTAGGGCACC CACCTGAAGG CACTTCCAGT TTCCAGCAGC AAGACTTTCC AGCATCTGCA GAGCTGGAGT TCTGCTCTCC TCTAAGCGAG ACCCTTACAA ACATACACAG CACTCTGCAG GGCTCCAATC GAACAAATAG AAGACTGAGA AGTGGATGCT GCTGGGCAGA AACGTGCCTG GCTTAGCAGA GGACAAACGA GTTAATCTTG CACCAGTCAC TCTGGCCCAA GAAGCCTATA GCTGGTGCAC TTGGGGCAAC ATAGACCCTA TAGACTTAGT AGCAATGATA GTATTCATAA TAATAGCTAA TGCTTACTGA ACACTCCCTG TGTGCCTGGC ACCTGCTAAG TATGTTATTT ACATTGTGTC ATTTAATCCT CGCAGTAGTC CTGTGGGTTA GATCTTACTA ATGTCATCAT TTTCAGATAA GTAAACAGAG GCACTGAGAG GTAGATCATA AGATCACACA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_002198, NM_001354924, NM_001354925, NR_149068, NR_149069
Synonyms	IRF-1; MAR
Summary	The protein encoded by this gene is a transcriptional regulator and tumor suppressor, serving as an activator of genes involved in both innate and acquired immune responses. The encoded protein activates the transcription of genes involved in the body's response to viruses and bacteria, playing a role in cell proliferation, apoptosis, the immune response, and DNA damage response. This protein represses the transcription of several other genes. As a tumor suppressor, it both suppresses tumor cell growth and stimulates an immune response against tumor cells. Defects in this gene have been associated with gastric cancer, myelogenous leukemia, and lung cancer. [provided by RefSeq, Aug 2017]

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