THEM5 (NM_182578) Human Mass Spec Standard

Specifications

Product Data
Description	THEM5 MS Standard C13 and N15-labeled recombinant protein (NP_872384)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC210954
Predicted MW	27.7 kDa
Protein Sequence	Sequence Link (showhide) >RC210954 protein sequence Red=Cloning site Green=Tags(s) MIRRCFQVAARLGHHRGLLEAPRILPRLNPASAFGSSTDSMFSRFLPEKTDLKDYALPNASWCSDMLSLY QEFLEKTKSSGWIKLPSFKSNRDHIRGLKLPSGLAVSSDKGDCRIFTRCIQVEGQGFEYVIFFQPTQKKS VCLFQPGSYLEGPPGFAHGGSLAAMMDETFSKTAFLAGEGLFTLSLNIRFKNLIPVDSLVVMDVEVDKIE DQKLYMSCIAHSRDQQTVYAKSSGVFLQLQLEEESPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_872384
RefSeq Size	1146
RefSeq ORF	741
Synonyms	ACOT15
Locus ID	284486
Cytogenetics	1q21.3
Summary	Has acyl-CoA thioesterase activity towards long-chain (C16 and C18) fatty acyl-CoA substrates, with a preference for linoleoyl-CoA and other unsaturated long-chain fatty acid-CoA esters (PubMed:22586271). Plays an important role in mitochondrial fatty acid metabolism, and in remodeling of the mitochondrial lipid cardiolipin (PubMed:22586271). Required for normal mitochondrial function (PubMed:22586271).[UniProtKB/Swiss-Prot Function]

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