PD1 (PDCD1) (NM_005018) Human Over-expression Lysate
CAT＃: LC401555
PDCD1 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
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CNY 900.00
| Cited in 3 publications. |
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CNY 1999.00
CNY 2700.00
CNY 600.00
Specifications
| Product Data | |
| Description | PDCD1 HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC210364 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 31.6 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_005009 |
| Locus ID | 5133 |
| Cytogenetics | 2q37.3 |
| Protein Families | WB |
| Protein Pathways | Cell adhesion molecules (CAMs), T cell receptor signaling pathway |
Citations (3)
| The use of this Proteins has been cited in the following citations: |
|---|
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Direct therapeutic targeting of immune checkpoint PD-1 in pancreatic cancer
,null,
British Journal of Cancer
,PubMed ID 30377341
[PDCD1]
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The prognostic role of immune checkpoint markers programmed cell death protein 1 (PD-1) and programmed death ligand 1 (PD-L1) in a large, multicenter prostate cancer cohort
,null,
Oncotarget
,PubMed ID 28460462
[PDCD1]
|
|
Assessing PDL-1 and PD-1 in Non-Small Cell Lung Cancer: A Novel Immunoscore Approach.
,null,
Clinical lung cancer
,PubMed ID 27816392
[PDCD1]
|
Documents
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PD-1 (PDCD1) mouse monoclonal antibody, clone OTI4F10 (formerly 4F10)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
