Vimentin Bovine Protein

Specifications

Product Data
Species	Bovine
Predicted MW	57,000
Concentration	lot specific
Purity	>98% determined by SDS gelelectrophoresis
Buffer	Presentation State: Purified State: Lyophilised purified protein Buffer System: 10 mM sodium phosphate pH 7.5, 6M urea, 2 mM DTT, 1 mM EDTA, 10 mM methylammonium chloride
Preparation	Lyophilised purified protein
Applications	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM sodium phosphate, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
Protein Description	Bovine vimentin
Note	Isoelectric Point: pI 5.3
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_776394
Locus ID	280955
Cytogenetics	13q16-q17
Synonyms	VIM
Summary	vimentin
Protein Families	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM sodium phosphate, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

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