Proteins
Vimentin human recombinant protein, 0.1 mg
| Expression Host | E. coli |
Vimentin bovine protein, 0.1 mg
Purified recombinant protein of Mouse vimentin (Vim), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
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- e.g. Dulbecco's PBS). (4)
- 10 mM sodium phosphate (2)
- 10mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
- 2mM dithiothreitol (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above) (2) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above) (2) - pH 7.4). For immunization purposes (2)
-
pH 7.4).
For immunization purposes (2) - protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl (2)
- the solution can be further dialyzed against PBS (Phosphate Buffered Saline (2)
- the solution can be further dialyzed against PBS (phosphate buffered saline (2)
