PRAS40 (AKT1S1) (NM_032375) Human Recombinant Protein
CAT#: TP300919
Recombinant protein of human AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 1, 20 µg
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Specifications
| Product Data | |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence |
>RC200919 representing NM_032375
Red=Cloning site Green=Tags(s) MASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPCAYPAHGRGALAEAARRCLHDIALAHR AATAARPPAPPPAPQPPSPTPSPPRPTLAREDNEEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDL PPFCESDPESTDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPP SSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Tag | C-Myc/DDK |
| Predicted MW | 27.2 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Reference Data | |
| RefSeq | NP_115751 |
| Locus ID | 84335 |
| UniProt ID | Q96B36 |
| Refseq Size | 2344 |
| Cytogenetics | 19q13.33 |
| Refseq ORF | 768 |
| Synonyms | Lobe; PRAS40 |
| Summary | AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC410168 | AKT1S1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC420655 | AKT1S1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY410168 | Transient overexpression lysate of AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 1 |
CNY 3080.00 |
|
| LY420655 | Transient overexpression lysate of AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 2 |
CNY 3080.00 |
|
| PH300919 | AKT1S1 MS Standard C13 and N15-labeled recombinant protein (NP_115751) |
CNY 19520.00 |
|
| PH311995 | AKT1S1 MS Standard C13 and N15-labeled recombinant protein (NP_001092102) |
CNY 19520.00 |
|
| PH312216 | AKT1S1 MS Standard C13 and N15-labeled recombinant protein (NP_001092103) |
CNY 19520.00 |
|
| TP311995 | Recombinant protein of human AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 2, 20 µg |
CNY 2900.00 |
|
| TP312216 | Recombinant protein of human AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 3, 20 µg |
CNY 2900.00 |
